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Pyrolysis Pyrolysis is a technique normally used to analyze nonvolatile organic compounds such as wood, paper, or polymers by GC/MS. In pyrolysis the sample is heated rapidly to 750 C or higher in order to thermally decompose it. A high temperature such as this is su cient to break the polymer backbone, forming smaller, more volatile fragments. By examining these fragments, it is sometimes possible to deduce the structure of the polymer chain. Some polymers unzip during pyrolysis to yield only the original monomer. This technique is used frequently to examine materials for the presence of additives such as plasticizers, antioxidants, ame retardants, ultraviolet (UV) stabilizers, or sizing treatments applied to cloth samples. Solids Probe Direct solids probe analysis is a volatilization technique that places the sample under vacuum near the mass spectrometer s ion source. The sample s temperature can either be raised to a preset maximum or it can be heated gradually, in a temperatureprogrammed fashion. During this time, the molecules that volatilize from the sample continuously enter the mass spectrometer s source and are ionized much in the same fashion as described above with GC/MS. The disadvantage of this technique is that there is no separation step. This is yet another way to analyze nonvolatile samples using mass spectrometry.

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Weyer, C, Bogardus, C, Mott, DM and Pratley, RE (1999) The natural history of insulin secretory dysfunction and insulin resistance in the pathogenesis of type 2 diabetes mellitus The Journal of Clinical Investigation, 104 (6), 787 94 Weyer, C, Funahashi, T, Tanaka, S et al (2001) Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia The Journal of Clinical Endocrinology and Metabolism, 86 (5), 1930 5 WHO expert consultation (2004) Appropriate body mass index for Asian populations and its implications for policy and intervention strategies Lancet, 363, 157 63 Widen, E, Lehto, M, Kanninen, T et al (1995) Association of a polymorphism in the beta 3adrenergic-receptor gene with features of the insulin resistance syndrome in Finns New England Journal of Medicine, 333, 348 51 Wofford, MR and Hall, JE.

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and P ( s ) may be interpreted as the local wavelength of the oscillation divided by 27r. The quantities a,p, and y are usually referred to as Courant-Snyder parameters collectively; from now on, the function p will be referred to as the amplitude function. So the general solution to the equation of motion can be written as

(2004) Pathophysiology and treatment of obesity hypertension Current Pharmaceutical Design, 10 (29), 3621 37 World Health Organization (1999) Department of Noncommunicable Disease Surveillance De nition, diagnosis and classi cation of diabetes mellitus and its complications World Health Organization (2005) Preventing chronic diseases a vital investment http://wwwwho int/chp/chronic_disease_report/en/indexhtml, (last accessed 1 June 2006) Wu, AY, Kong, NC, de Leon, FA et al (2005) An alarmingly high prevalence of diabetic nephropathy in Asian type 2 diabetic patients: the MicroAlbuminuria Prevalence (MAP) Study Diabetologia, 48, 1674 5 Xia, Z, Sniderman, AD and Cian one, K (2002) Acylation-stimulating protein (ASP) de ciency induces obesity resistance and increased energy expenditure in ob/ob mice The Journal of Biological Chemistry, 277 (48), 45874 9 Xiang, K, Wang, Y, Zheng, T et al.

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is to change the pH or ion strength by applying a stationary phase with a different buffer composition. For very strongly bound samples, the use of chaotropic reagents such as urea may have to be considered. However, the use of harsh conditions may lead to loss of biological activity of the protein to be puri ed. The likely strength of binding and speci city of the molecule to be puri ed for the immobilized ligand are important criteria in the design of the stationary phase to be used in this technique. A gentler approach is to elute the bound molecule with an excess of the af nity ligand in the mobile phase. Soluble and immobilized versions of the ligand compete with each other for the binding site on the protein, thus gently eluting it from the stationary phase. A variation of this approach which is useful in situations where closely related proteins (e.g. isoenzymes) have bound to a single immobilized ligand is to create an af nity gradient,

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(2004) Genomewide search for type 2 diabetes/impaired glucose homeostasis susceptibility genes in the Chinese: signi cant linkage to chromosome 6q21 q23 and chromosome 1q21 q24 Diabetes, 53, 228 34 Xu, JY, Dan, QH, Chan, V et al (2005) Genetic and clinical characteristics of maturity-onset diabetes of the young in Chinese patients European Journal of Human Genetics, 13, 422 7..

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